桿体外節の分離

概要

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A new method for isolating the rod outer segments from retinal tissues was devised. Being suspended in Ringer solution, the rod outer segments isolated by the sucrose method are immediately deformed and eventually take more or less extended forms. in order to improve this point, the author tried to isolate the rod outer segments by use of erythrodextrin Ringer solution (s. g. 1.10 and 1.07) instead of sucrose solution. The rod outer segments thus prepared maintain thier normal forms even when suspened in Ringer or 5% polyvinylpyrrolidone Ringer solution. This method has proved that the specific gravity of the rod outer segments is 1.08-1.09 and that the difference spectrum of the suspension of the rod outer segments is quite similar with that of rhodopsin solution
社団法人日本動物学会の論文
1957-10-15