ユムシUrechis unicinctus (von Drasche)における受精の研究 : (第II報)卵膜および受精膜に対する尿素の作用
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Chase ('35) reported that in an attempt to dissolve the membranes of the Urechis caupo eggs, not only their fertilization membrane but also their vitelline membrane are not easily soluble in 1 M urea solution, and that both fertilization and vitelline membranes do not come to dissolve in any solutions until they are soaked in urea solution for a long time such as from 15 to 24 hours. The author recently performed the same kind of experiments on the eggs of Urechis unicinctus and obtained the following results: (1) The vitelline membrane dissolves in 1 M urea solution (pH 8.2) for a comparatively short time, namely, from 40 minutes to 2 hours. Time is more necessary for the membrane to be dissolved when the urea solution is weak acid or neutral, or the solution is kept at lower temperature (Table 1. A & B). (2) The vitelline membrane, during the time of dissolution, becomes swollen and breaks up into two layers, inside and outside. In most of the eggs, then, the external thin membrane begins to dissolve and next occurs the disappearance of the internal thin membrane. However, in a considerble number of eggs, the two thin membranes completely disappear at the same time (Fig. 1). (3) The fertilization membrane also dissolves in 1 M urea solution (pH 8.2) through almost the same process as the vitelline membrane. The fertilization membrane has not necessarily stronger resistance against the urea solution than the vitelline membrane. The time required for dissolution is about the same(Fig. 2). Even in the dissolution of the fertilization membrane, time is more needed provided the urea solution is weak acid or neutral, or the solution is kept at lower temperature. (4) Dissolution of the vitelline and fertilization membranes by urea solution is either hindered or inhibited by means of adding appropriate quantities of inorganic, isotonic salt solutions as NaCl, KCl, and CaCl_2 to the urea solutions. Addition of only a small amount of CaCl_2 solution shows a strong hindrance or inhibition (Table 2).
- 社団法人日本動物学会の論文
- 1956-05-15
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- ユムシUrechis unicinctus (von Drasche)における受精の研究 : (第II報)卵膜および受精膜に対する尿素の作用