ウニ卵のアミノ・アシルRNA(予報)

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Amino acid incorporation by cell free systems of sea urchin eggs begins to increase after fertilization (Hultin 1961). Wilt and Hultin (1962), Nemer (1962) and Nemer and Bard (1963) demonstrated that poly-U could stimulate incorporation of phenylalanine by ribosomal or homogenate systems. This fact indicates that the limiting factor in unfertilized eggs is probably messenger RNA rather than some deficiency in the enzyme systems involved in protein synthesis. It is of importance, therefore, to investigate each step of protein synthesis e. g. amino acyl RNA formation with pH 5 enzyme and the transformation of amino acid from amino acid RNA to protein. Findings in the present study showed that a pH 5 enzyme of unfertilized eggs produced ^<14>C-aimino acyl RNA with almost the same specific activity as that of fertilized eggs (Table 1). Using ECTEOLA cellulose column chromatography, tha RNA extracted from the pH 5 enzyme of fertilized eggs incubated with ^<14>C-amino acids showed little change from that of unfertilized eggs (Fig. 1). The amino acid activating enzymes may change little at the time of fertilization. In contrast, activity of amino acid transfer enzyme was markedly different before and after fertilization (Fog. 2). There was no difference between the amino acyl RNA with respect to their properties as substrate for the transfer enzyme produced with pH 5 enzyme from unfertilized eggs and that from fertilized eggs, but the cell free system of fertilized eggs only could transfar amino acid into protein from amino acyl RNA. It is clear from these investigations that the only change taking place at the time of fertilization may be ribosomal. Furthermore, it is this change which may be responsible for the difference in the rate of protein synthesis in eggs before and after fertilization.
社団法人日本動物学会の論文
1963-09-15

ウニ卵のアミノ・アシルRNA(予報)

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