ムササビ卵の仁の細胞化学的研究
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概要
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Cytochemical studies were made of the nucleoli found in the ovarian tissues of a young female Japanese large flying squirrel (Petaurista leucogenys) obtained from a small mountain near Koi, Hiroshima City, Japan. The ovaries were fixed in two kinds of fixatives for 37 hours around 20℃ (at room temp.): one was NAWASCHIN's fluid and the other CHAMPY's. A modified method of BORREL's staining technique was used with safranin used in place of fuchsin. In order to detect DNA, FEULGEN reaction after STOWELL (1945) was applied. Treating the ovarian tissues fixed in NAWASCHIN's fluid with the modified staining method resulted in nucleoli of numerous first oocytes turning sulphate green (hue 13-luminosity 16-saturation 5). However, in some oocytes (2.46%) located in the central protion of the slice of tissue, another type of nucleolus which had been colored Castilian red (1-15-9) was found in company with the sulphate green one. Judging from the studies previously made by the author, the red colored nucleolus is a karyosome and the green one is a plasmosome. It is suggested that karyosome in the oocytes when treated with NAWASCHIN's fluid as long as 37 hours are liable to dissolve, which explains why karyosomes were not found at the marginal portion of the slice of tissue. The FEULGEN reaction resulted in the nucleolus (karyosome) coloring a pale orchid (22-18-3). This fact suggests that a large amount of DNA in the karyosome was dissolved in the fixative. In the first oocytes fixed in CHAMPY's fluid, a constricted nucleolus was observed. One part of the nucleolus was stained red germanium (15-14-10), and the other part Corinthian purple (24-15-6). But these nucleoli assumed a negative FEULGEN reaction. Nucleoli in the oogonia, the follicle cells, or the mucosa cells of oviduct could be colored quite stronngly by safranin but not by indigocarmine after the action of NAWASCHIN's fluid or CHAMPY's. The FEULGEN reaction on these nucleoli were positive. A remarkable contrast can be seen in the comparison of nucleoprotein components of the nucleoli of the first oocytes with those of the oogonia, the follicle cells, or the mucosa cells of the oviduct. It was found that there was a wide difference between the nucleoprotein-components in the nucleoli of the first oocytes of the Japanese large flying squirrel and those of the Japanese common oyster Crassostrea gigas. The nucleoproteins of the karyosome in the oyster preserved well NAWASCHIN's fluid, but those of the large flying squirrel were liable to dissolve. Regarding the composition of the nucleoli in the nucleus, the author differs from the articles of CASPERSSON (1941), VINCENT (1955) and AMANO (1958). In the present studies, it may be stated that the nucleolus consists of only karyosome in the oogonium stage, while in the first oocyte, stage, the nucleolus consists of karyosome and plasmosome which developed from karyosome during the change from the oogonium stage to the first oocyte stage. It may be supposed that a nucleolus is changeable in both its morphological structure and its chemical components as the nucleolus develops and differentiates its function.
- 社団法人日本動物学会の論文
- 1962-07-15
著者
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