ウニ胚単離小割球の in vitro 培養によって形成された骨の形態について

概要

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Descendants of micromeres isolated at the 16-cell stage form skeletons in vitro, some of which are very similar to one of a pair of normal pluteus skeletons (Okazaki, 1971). It has not been known, however, whether such unilateral skeletons in vitro have a three-dimensional structure corresponding to right or left skeleton in vivo. To clarify this point, micromeres were isolated from Pseudocentrotus depressus and Hemicentrotus pulcherrimus embryos and cultured in sea water containing horse serum. After 3-4 days of culture, three-dimensional skeletons were chosen, and close examination was made on individual skeletons about positional relationships between three skeletal rods corresponding to the body, ventral transverse and antero-lateral rod of the pluteus skeleton. The number of right and left skeletons was almost equal in both P. depressus and H. pulcherrimus. During the observation on H. pulcherrimus, irregular skeletons were occasionally found which had two or three rods representing characteristic thickening of the body rod of the pluteus skeleton. These facts support the idea that division of labor exists among the descendants of the micromeres in an embryo in the shaping of the skeleton.
社団法人日本動物学会の論文
1977-06-25