プラナリアDugesia japonicaの基底膜に関する電子顕微鏡的研究

概要

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The subepidermal membrane of Dugesia japonica, a Turbellarian species, was studied by means of electron microscopic cytochemistry. The planarian subepidermal membrane consists of three structural elements: an electron-lucent zone facing the basal surface of the epidermal cell membrane, a microfibrillar layer occupying the major part of the subepidermal mambrane and a limiting layer which lies between these elements. The electron-lucent zone included an osmiophilic dense network which often showed a striped pattern. The netowrk was arranged with extremely thin filaments and particles. Bothe components of this network showed a strongly positive PTA reaction which may indicate the presence of neutral polysaccharides, but no reaction for acid mucopolysaccharide. The limiting layer was about 30nm in thickness and recognized as the accumulation of a moderately dense amorphous substance which showed a heavily positive reaction for acid mucopolysaccharide. This layer was found to connect with the epidermal cell directly through hemidesmosomes. The microfibrils (9 to 13nm in diameter) showed a moderate density in routine staining with uranyl acetate and lead citrate. Stained with PTA, electron density of the microfibrils was evidently increased. Moreover, ruthenium red staining revealed positive material at the surface of each microfibril. The diameter of the microfibril, therefore, appeared to come to 20nm.
1975-06-25