淡水産貝類精子の細胞化学的研究

概要

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By means of light and electron microscope cytochemistry, evidence is presented for the existence of some glycolytic and oxidative enzymes in the mature spermatozoa of the pond snails Sinotaia histrica and Heterogen longispira, and the freshwater mussel Hyriopsis schlegelii. Sites of activities of the enzymes are as follows: 1. Phosphorylase end-product appears on the surface around the glycogen granules in the tails of the snails spermatozoa. 2. Lactate dehydrogenase activity is absent from the tails of the snails spermatozoa but appears in the mitochondria where no glycogen granules are present. 3. Using copper ferricyanide reagent for the demonstration of succinate dehydrogenase activity, reaction products appear on the cristal membranes, in the intracristal spaces and in the outer compartments of the mitochondria of the snails and the mussel spermatozoa. 4. Using 3, 3'-diaminobenzidine tetrahydrochloride, cytochrome oxidase activities are localized on the cristal membranes and in the intracristal spaces of the mitochondria of the snail and mussel spermatozoa.
社団法人日本動物学会の論文
1974-06-25