ゾウリムシにおける接合型変化のリズムと細胞分裂

概要

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The circadian rhythm of mating-type change in cyclic stock of Paramecium multimicronucleatum, syngen 2, was studied. Cells grown with excess food at 25℃ were entrained to 12L-12D cycles (L=1000lux, day-light fluorescent lamp) for two weeks before experimentation. After exhausted the available food, they were kept in constant dim light (less than 1lux). At six hour intervals after the transfer to the dim light condition, a part of this culture was taken and supplied with an equal volume of the fresh culture medium. Thus the sequent cell division was observed after 12-18 hours of food supply. Thereafter, its mating reactivity was tested at every three hours and recorded in five grades if positive, regarding the maximal reactivity of mating type III as a peak of the rhythm. The result was that the peak of the circadian rhythm constantly persisted regardless of the occurence or the time of the fission.
社団法人日本動物学会の論文
1972-06-25