Amoeba proteusの親水性, および疎水性台床に対する付着について

概要

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For the purpose of revealing the mechanism of adhesion to substrata in Amoeba proteus, adhesion ratios (adhered no./total no.) to the hydrophobic substrata (polystyrene, polyethylene, parafilm) and the hydrophilic ones (glass, agar, cellulose acetate) were obtained in the materials incubated in inorganic culture medium or in buffered solutions at various pH. In the case of 300 minutes incubation in inorganic culture medium (pH 5.4, I=1.0×10^<-4>), adhesion ratio of non-treated amoebae to glass (0.67±0.09) was lower than that (about 1) to other substrata. Using with amoebae pretreated in redistilled water for 20-25 minutes, adhesion ratios to polystyrene, polyethylene, parafilm and 2% agar were about 0.9 within 60 minutes. However, the ratios to glass and cellulose acetate were about 0.5 after 120 minutes incubation. The adhesion ratio to glass was affected intensely by the degree of pH and was increased very fast within the range of pH 5.8-7.8. However, the more alkalinity rose, the more adhesion ratio dropped fast after long time incubation. On one side, effect of pH on adhesion ratios to polystyrene, parafilm and agar were not so remarkable as to the case of glass. Based on these experimental results, it was discussed that non-specific adhesion of amoeba to substrata might be caused by the total of electrostatic forces of repulsion, van der Waals-London force and another attractive one.
社団法人日本動物学会の論文
1971-04-15