Influence of the Preimplantation-Embryo-Development(Ped) Gene on Mouse Blastocyst Differentiation
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概要
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Mouse preimplantation embryonic cleavage rate is dependent upon the presence or absence of the Preimplantation-embryo-development(Ped)gene ; which is linked to the Qa-2 subregion of the H-2 complex. Expression of Qa-2 antigens by fast developing mouse embryos correlates with Ped gene pheno-type : Qa-2^a. It is not known if the Ped gene(Qa-2^a)participates in cell differentiation in the preimplantation mouse blastocyst. Therefore, the study objective was to determine the differentiation of cells to the inner cell mass(ICM)and trophectoderm(TE)in Qa-2^a positive(Ped+)and Qa-2^a negative(Ped-)mouse blastocysts. One-cell stage embryos were recovered from the excised oviducts of PMSG(5 IU)and hCG(5 IU)primed virgin female(3-4 weeks)BALB/cByJ(Qa-2^a : Ped-)and BALB/cJ(Qa-2^a : Ped+)mice mated to fertile males(12+ weeks). Embryos were collected, 14 hr after hCG, and cultured in modified α-MEM, to the hatched blastocyst stage in an atmosphere of 5% CO_2 in air, 95% relative humidity at 37℃. Cell differentiation was determined by differential staining(bis-benzimide and propidium iodide)and fluorescence microscopy. Data were analyzed by Students t-test. There was no significant difference in total cell number between BALB/cJ(mean 139)and BALB/cJ(mean 143)embryos. A significant difference(p<0.001)was found in the number of cells differentiating to the ICM between BALB/cJ(mean 59.0)and BALB/cByJ(mean 29.9)mouse embryos. The number of cells differentiating to the TE, between BALB/cJ(mean 80.0)and BALB/cByJ(mean 114)embryos, approached significance(p=0.062). The results suggest that the Ped gene(Qa-2^a)may have an influential role in preimplantation blastocyst cell differentiation. Additional studies are warranted to further elucidate the role of the Ped gene in preimplantation embryo development and blastocyst formation.
- 社団法人日本動物学会の論文
著者
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Roudebush W
Division Of Reproductive Endocrinology Department Of Obstetrics And Gynecology Medical University Of
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Roudebush William
Division of Reproductive Endocrinology, Department of Obstetrics and Gynecology, Medical University