An Open Cephalic Neural Tube Reproducibly Induced by Cytochalasin D in Rat Embryos in vitro
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概要
- 論文の詳細を見る
Rat embryos at the head-fold stage (Slc : SD strain ; 9. 5 days of gestation) were cultured in rat serum in the presence of a relatively low concentration of cytochalasin D (2×10^<-8> M). Embryos developed into C-shaped structures (with the open part of the C directed ventrally) with an open cephalic neural tube. Elevation and apposition of the neural folds and eversion of the neural plates at the procencephalon, mesencephalon and some parts of rhombencephalon were observed during the course of development of the treated embryos. In control embryos, staining with rhodamine-conjugated phalloidin was observed at the basal region of the lateral margin of the fusing neural folds and at the luminal surface of the roof plates of the procencephalic neural tube. The latter diminished as embryos developed. However, the staining was confined to the edges of the apposed neural folds in the treated embryos and became intense at the luminal surface of the everted neural plates. By contrast, some parts of the rhombencephalon of the treated embryos showed fusion of the neural folds. The staining was observed in roof plates of the rhombencephalon in these embryos as like as control embryos. The area of the staining at the luminal surface of the roof plates spread as the fourth ventricle expanded. These results suggest that microfilaments do not play an essential role in the elevation of the neural plates but do play an important role in fusion of the neural folds and the moulding of the cephalic neural tube. Delicate changes in the distribution of microfilaments may result in changes in cell shape that cause the fusion of the neural folds and the moulding of the cephalic neural tube.
- 社団法人日本動物学会の論文
- 1994-08-15
著者
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Keino H
Inst. Developmental Res. Aichi Jpn
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Keino Hiroomi
Perinatology Institute For Developmental Research
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Matsuda Motoko
Department of Embryology, Institute for Developmental Research, Human Service Center
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Matsuda Motoko
Departments Of Embryology Institute For Developmental Research
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