K252a , a Potent Inhibitor of Protein Kinases, Promotes the Transition of Dictyostelium Cells from Growth to Differentiation

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概要

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• Cell differentiation and proliferation are mutually exclusive processes in many cases. The transition of starving Dictyostelium cells from growth to differentiation phase has been shown to occur at a particular position (putative shift point ; PS-point) in the cell cycle of D. discoideum Ax-2. The significance of phosphorylation states of proteins such as 101 kDa, 90 kDa, and 32 kDa phosphoproteins has been argued, particularly around the PS-point. In this study we examined effects of the protein kinase inhibitors and activators on the transition of Ax-2 cells from growth to differentiation . K252a , a potent inhibitor of protein kinases , inhibited growth possibly through the blockage of pinocytotic activity of cells, and promoted the progress of development after starvation when applied to Ax-2 cells at the growth phase . Such a K252a-effect was most pronouncedly exhibited on the cells located near the PS-point. Unexpectedly , however, the development of starved cells was found to be considerably delayed by staurosporine bearing a structural and functional resemblance to K252a when it was applied during the growth phase. Pulse-labelings of growing Ax-2 cells with inorganic ^<32>P (^<32>Pi) showed that K252a induces the disappearance of a 48 kDa phosphoprotein and the appearance of a 50 kDa phosphoprotein, specifically in the cells located around the PS-point. Phosphorylation of 32 kDa and 24 kDa proteins was also inhibited by K252a, but this inhibition was not necessarily specific to the K252a-treatment and occurred independently of the cell-cycle phases. The possible significance of these results is discussed in relation to a breakaway of cells from proliferation to differentiation at the PS-point.

• 社団法人日本動物学会の論文
• 1994-02-15

著者

• Maeda Y

  Kochi Univ. Kochi

• MAEDA Yasuo

  Biological Institute, Faculty of Science, Tohoku University

• Furukawa Takeshi

  Biological lnstitute, Faculty of Science, Tohoku University

• Furukawa Takeshi

  Biological Lnstitute Faculty Of Science Tohoku University

• Maeda Yasuo

  Biological Institute Faculty Of Science Tohoku University

関連論文

• Specific expression of a gene encoding a novel calcium-binding protein, CAF-1, during transition of Dictyostelium cells form growth to differentiation
• Dictyostelium mucoroides cells exhibit cell cycle-dependent sorting behavior as observed in D. discoideum development
• K252a , a Potent Inhibitor of Protein Kinases, Promotes the Transition of Dictyostelium Cells from Growth to Differentiation
• Cell-cycle progression during the development of Dictyostelium discoideum and its relation to the subsequent cell-sorting in the multicellular structures
• Overexpression of CAF1 encoding a novel Ca^-binding protein stimulates the transition of Dictyostelium cells from growth to differentiation
• A Proteinous Factor Mediating Intercellular Communication during the Transition of Dictyostelium Cells from Growth to Differentiation
• Rapid patterning in 2-D cultures of Dictyostelium cells and its relationship to zonal differentiation
• Rapid patterning of Dictyostelium discoideum cells under confined geometry and its relation to differentiation
• Mutual Relation between the Cell-Cycle Progression and Prespore Differentiation in Dictyostelium Development
• Relevance of Histone H1 Kinase Activity to the G2/M Transition during the Cell Cycle of Dictyostelium discoideum

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