An Extraction Method of Dynamic Features in Pulsing Organs of Caenorhabditis Elegans During Feeding

概要

論文の詳細を見る
Caenorhabditis elegans during feeding gives good "moving biological images", in which motions of several pulsing organs are superposed on its head swing. A powerful method to extract dynamic features is presented. First step is to use a variance picture VAG4 in order to pick up active pixel coordinates of concerned moving objects. Superiority of VAG4 over usual variance picture VAG2 is shown quantitatively by a model of moving particles. Pulsing areas of C. elegans, are exhibited more clearly in VAG4 than VAG2. Second step is use of a new subtraction method to extract main frequency bands. FFT spectra are averaged in active positions where VAG4 is above threshold THVR in the square with 8×8 pixels (ONA). The power spectra averaged in the enlarged squares (ELA) are subtracted from those in ONA, in which ELA includes ONA in its centre position. Large peak bands emerge in the subtracted power spectra. The subtraction eliminates the effect of head swing by spatial averagings in ELA. This new emphasizing method is compared to another subtraction method. The characteristic frequency of periodical moving organs coincides well with the values observed by other research groups and our visual estimation of replayed VTR images. Thus the proposed extraction method is verified to work well in double superposed motions.
社団法人電子情報通信学会の論文
1996-01-25