Visualizing the Signal Transduction Pathways in Living Cells with GFP-Based FRET Probes

スポンサーリンク

概要

• 論文の詳細を見る

• Visualizing how signals are transmitted within a living cell has long been a goal of molecular biologists, which has now been realized by probes based on the principle of fluorescence resonance energy transfer (FRET). Variants of green fluorescent protein (GFP) enabled the preparation of genetically-encoded FRET probes, and their application has been expanded for use in many areas of biology. The GFP-based FRET probes can be classified as belonging to one of two types, intermolecular and intramolecular FRET probes. The merit of the intermolecular FRET probe lies in the ease of preparation of the probes, whereas the merit of the intramolecular FRET probe lies in the high signal-to-noise ratio. Although these GFP-based probes are powerful tools for the visualization of signal transduction cascades, numerous pitfalls remain associated with this technique. Here, we provide an overview of the GFP-based FRET probes and discuss these issues.

• 日本組織細胞化学会の論文

著者

• Kurokawa Kazuo

  Department Of Tumor Virology Research Institute For Microbial Diseases Osaka University

• Matsuda Michiyuki

  Department Of Pathology And Biology Of Diseases Kyoto University Graduate School Of Medicine

• Takaya Akiyuki

  Department of Tumor Virology, Research Institute for Microbial Diseases, Osaka University

• Terai Kenta

  Department of Tumor Virology, Research Institute for Microbial Diseases, Osaka University

• Fujioka Aki

  Department of Tumor Virology, Research Institute for Microbial Diseases, Osaka University

• Terai Kenta

  Department Of Tumor Virology Research Institute For Microbial Diseases Osaka University

• Takaya Akiyuki

  Department Of Tumor Virology Research Institute For Microbial Diseases Osaka University

• Terai Kenta

  Department Of Cell Biology National Cerebral And Cardiovascular Center Research Institute

• Fujioka Aki

  Department Of Tumor Virology Research Institute For Microbial Diseases Osaka University

関連論文

• Blo-imaging of the activation of signaling molecules and cytoskeletal molecules
• Epidermal Growth Factor-dependent Dissociation of CrkII Proto-oncogene Product from the Epidermal Growth Factor Receptor in Human Glioma Cells
• Fluorescence (Forster) resonance energy transfer imaging of oncogene activity in living cells
• Endogenous tenascin-C enhances glioblastoma invasion with reactive change of surrounding brain tissue
• Visualizing the Signal Transduction Pathways in Living Cells with GFP-Based FRET Probes
• Heat Shock Proteins Regulates Cardiomyocytes via Toll-Like Receptors

スポンサーリンク