A NEW METHOD FOR GEOMETRICAL DEMONSTRATION OF LDH ISOENZYMES : HISTOCHEMICAL ELECTROPHORESIS
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概要
- 論文の詳細を見る
This paper describes a new method for the geometrical demonstration of lactate dehydrogenase (LDH) isoenzymes in tissues and organs (histochemical electrophoresis). The principle of this method can be found in the following points. (i) The tissues obtained from various organs were frozen, and cut in 8 μ slices with a cryostat. The sections were placed on a piece of filterpaper and dried immediately under a fan. (ii) As a result, the enzyme molecules of the tissue were resorbed in the surface layer of the filter paper. (iii) Then, the filter paper is treated by standard electrophoresis. The data obtained with this new method were compared to those of tissue extracts of the heart, liver and kidney of dogs. This new method makes it possible to investigate the distribution of isoenzymes in tissue as a whole. Therefore, it may enable us to clarify the geometrical distribution of LDH isoenzymes in various organs.
- 日本組織細胞化学会の論文
著者
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Yamasawa Ikuhiro
The 2nd Dept. Of Intern.med. Tokyo Medical College
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NOHARA YOSHITSUGU
The 2nd Dept. of Intern. Med., Tokyo Medical College
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Yamasawa Ikuhiro
The 2nd Dept. Of Intern. Med. Tokyo Medical College
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Nohara Yoshitsugu
The 2nd Dept. Of Intern. Med. Tokyo Medical College
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Nohara Yoshitsugu
The 2nd Department Of Internal Medicine Tokyo Medical College
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Yamasawa Ikuhiro
The 2nd Department Of Internal Medicine Tokyo Medical College
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