THE EFFECTIVENESS OF OUR COLLOIDAL IRON METHOD IN THE ELECTRON-HISTOCHEMICAL DEMONSTRATION OF SULFHYDRYL AND DISULPHIDE WITH PARTICULAR REFERENCE TO PANCREATIC ZYMOGEN GRANULES

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The colloidal iron method here introduced is an improved form of the Chevremont-Frederic method for the SH+SS groups. The principal point of improvement is the use of Muller's colloidal iron hydroxide instead of ferric sulphate as the ferric iron reacting with the ferrocyanide ion. This modification significantly reduced staining contamination, significantly increasing staining contrast; therefore, it made to be possible the electron micro scopical demonstration of the protein bound SH+SS groups. However, strict care must be taken against non-specific reduction caused by the tissue components and their adsorption reactivity. In the present experiment, this method was applied to the electron microscopic demonstration of the SH+SS groups in rat pancreatic acini, in which the internal portion and the limiting membrane of the secretory granules yielded clearly positive results.
日本組織細胞化学会の論文