The Automatic Thin Sectioning and Staining System for Light Microscopy : The Tests by Two Trial Products (<Workshop III>Three-dimensional Microscopy Combined with Rapid Automatic Tissue Preparation Techniques)

概要

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The preparation of tissue sections for light microscopy is absolutely necessary in pathology. However, this process is still mainly performed manually. The preparation for many samples from a large number of specimens requires meticulous skills. In addition, it is very difficult to maintain a constant accuracy of the producedure. To solve these problems we propose two new test machineries for autmatic highspeed sample preparations. The first apparatus is conceptually based on attaching pressure-sensitive adhesive tape on the surface of a paraffin block prior to sectioning. This whole process could be repeated automatically. The sectioning is performed by a newly developed high-precision automatic microtome. Using the proposed machine, cut sections could be continuously mounted on the tape and stained automatically. We also developed the second machine to mount sections on the object glass automatically by using static electricity. The principle of this method is based on electrostatically charging the surface of a paraffin block by which insulation tape was attracted and attached to the surface of the block. After sectioning the block, the section remains adhered on the insulation tape by electrostatic forces. Subsequently, the section is transferred to the object glass by using static electricity as well. In the use of such method, tissue section on conventional object glass could be made fully automatic.
日本組織細胞化学会の論文