蓚酸カルシウム結晶の成長を抑制する高分子物質についての研究 : 第1編 : 蓚酸カルシウム結晶成長の測定法ならびに尿中各種抑制物質の活性について

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1) A simple and reliable method was developed for assay of calcium oxalate crystal growth. A metastable solution of CaCl_2 (1 mM) and sodium oxalate (0.2 mM) in 0.05 M barbiturate-acetate buffer, pH 5.7, NaCl 0.15 was seeded with calcium oxalate monohydrate crystals (0.06 mg/ml). Tubes (12 ml) were stirred and, at 40 min., aliquots were removed and filtered through Millipore filters (0.22 μm). The ^14C radioactivity was determined in the filtrate, and the ratio of count to control, preseeding , was used to estimate the fraction of oxalate that had been lost from the liquid phase and had entered the solid phase by combining during growth of the seed crystals. The validity of this method was confirmed by many experiments. 2) Non-dialysable, high molecular weight inhibitor (s) in human urine contribute about half or more than half of the calcium oxalate crystal growth inhibitory activity of whole urine. 3) Although magnesium is weaker than inorganic pyrophosphate and citrate at the concentrations usually present in urine, its potency increases greatly when its concentration increases.
社団法人日本泌尿器科学会の論文
1978-10-20