血尿の研究 : (第1篇)血尿の血液濃度判定に關する實驗的研究並に簡易比色測定装置製作の理論
スポンサーリンク
概要
- 論文の詳細を見る
Hematuria is an important clinical sign encountered not only in urological practice but also in almost all other clinical fields, and yet practically no attempts have been made for estimating the degree of hematuria based on precise criteria for its expression. Consequently, the statements found in medical literature as to the severity of hematuria are extremely in adequate and the limits of danger as to life as indicated by the extent of hematuria have not been clearly defined. This is because of numerous difficulties involved in this problem in spite of its apparent simplicity. For the purpose of expressing hematuria on a more quantitative basis, the author has established certain standards for judging the digree of hematuria in terms of heme concentration. This was done by first converting the blood present in various hematuric specimens into HbO_2 or HiCN and the data so obtained were plotted in the form of a nomogram. By this means the amount, of blood passed in the urine was roughly estimated. This principle has furthermore, been applied to the construction of a simple colorimeter, in which the color developed in the urine specimen was compared with the colored glass filter corresponding to the known heme concentration. The details of the steps involved in this method are as follows: . 1) The blood present in a hematuric sample is first converted into HbO_2 or HiClN and from the values of absorption maxima actually determined by spectrophotometry a nomogram is constructed, which enables one to find the blood concentration in terms of heme. A series of standards for estimating the extent of hematuria was set up on the basis of heme concentration. By ascertaining the tinctorial characteristics of the HbO_2 and HiCN solutions, the standard solution:s and the colored glass filters for use in the contruction of colorimeter have been precisely determined. 2) In the quantitative determination of hemoglobin derivatives, the increase in the content of MetHb due to the therapeutic use of phenacetine or sulfonamides has attracted Wuch attention. In cases of hematuria due to the bleeding from the upper urinary tract or after retention in the bladder for a definite period of time, the degree of methemoglobinuria must necessarily differ from the condition obtaining in the blood stream. With reference to artificial hematuria, some experiments have been performed in vitro as well as by means of rabbit bladder. The results indicated that the amount of methemoglobin formation increases with the lapse of time but it is generally slow during the first 4 hours. Thus, although the Hb.derivatives can be estimated by the HbO_2 method, the most accurate can be obtained by means of the HiCN method from the samples which remained in the bladder for a period longer than 4 hours.
- 社団法人日本泌尿器科学会の論文