睾丸におけるゴナドトロピンの作用機序に関する免疫組織学的研究 : 第II報ヒト絨毛性ゴナドトロピン投与後の睾丸内局在について

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Our previous study demonstrated that endogenous LH was localized mainly in immature Leydig cells and only a few mature Leydig cells of human testis using immunohistochemical techiniqes. However, futher study is necessary to determine the exogenous LH (hCG) binding sites in human testicular tissue. Ten patients were injected with hCG (5000 IU) i.m. before urological surgery, and testicular tissues were obtained at different times during scrotal manipulation. The hCG localization in human testicular tissues was investigated by means of indirect tests of immunofluorescence or immunoperoxidase technique in terms of FITC or HRP labelled IgG, respectively. Positive stained cells appeared in numbers of immature Leydig cells even after hCG injections. Especially 3 hours after injection, there are many positive cells which are composed of immature and mature Leydig cells. Another finding suggest that hCG could be permeated into seminiferous tubules. However, at 4 hours positive cells in the interstitial tissue decreased in number and positive labelled materials could not be seen in the intratubular space. All positive labelled materials were localized only in the cytoplasm.