末梢炎症性疼痛における局所脊髄ブドウ糖代謝率およびプロテインキナーゼ C 結合の変動
スポンサーリンク
概要
- 論文の詳細を見る
The functional implication of complicated anatomical and neurochemical events of the spinal cord has been progressibly clarified. Although there are many evidences implicated as the primary sensory transmitter of acute pain in spinal cord, the reasons why the accelerates is not clear. We attempted to clarify the changes in local spinal cord glucose utilization (LSCGU), and protein kinase C (PKC) activation/translocation by MO (mustard oil) injection in relation to pain behaviour. Fourteen male Wistar rats were subjected to MO testing. The flinching of the injured paw was counted every 5min for the duration of 60min period. For the determination of LSCGU, ^<14>C-2-deoxyglucose (^<14>C-2DG) method was performed in the spinal cord at the same level as the 2^<nd> and 5^<th> cervical vertebrae for 60 min after MO injection. Simultaneously, using same frozen tissue, the binding sites of ^3H-phorbol-dibutylate (PDBu) for PKC activity were determined by means of quantitative in vitro autoradiography. Following results were obtained : 1. The number of flinching after MO injection increased significantly in the MO group more than in the control group during 15min to 60min period. 2. ^<14>C-2DG was increased significantly at laminae I-II and V-VI in the ipsilateral spinal cord at the same level as the 5^<th> cervical vertebrae in the MO group than in the contralateral spinal cord in the MO group and bilateral spinal cord in the control group. 3. Bindings for ^3H-PDBu at laminae I-II in the spinal cord were the densest, and binding at laminae I-II and V-VI in the ipsilateral spinal cord at the same level as the 5^<th> cervical vertebrae in MO group than in the contralateral spinal cord in MO group and bilateral spinal cord in control group. The present study revealed the hyperalgesia in inflammatory pain by mustard Oil injection caused the spinal sensitization by the increase in LSCGU and the activation of PKC.
- 九州歯科学会の論文
- 2001-04-25