ラット顎下腺部分切除後の創傷治癒に関する実験的研究
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概要
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In daily clinical work, at the time of enucleation of salivary calculus in sialolithiasis or of a mucous cyst occurring on the oral floor, injuries to the salivary gland tissue are often experienced. At present, however, histological characteristics of repair and regeneration of severed or injured salivary gland have not yet been clarified sufficiently. Yet, it is important to have a sufficient grasp of effects of various surgical operations on salivary gland tissue and healing process. Partial extirpation of the submandibular glands of Wistar rats was performed and an investigation of wound healing and effects on the remaining salivary glands was conducted. The results were as follows : 1. The portion near the excretory duct of the submandibular gland of Wistar rats was partially extirpated and the healing process of wound was investigated. At the early stage, persistence of blood clots, formation of fibrin, and inflammatory cell infiltration were observed. Thereafter, increase with time in glannulation tissue and then replacement by fibrous scar tissue occurred. II. Remaining salivary gland tissue A. In the terminal portion, atrophy, destruction, disappearance, pyknosis, and deformation of acinous cells were seen, and along with these regressive changes, decrease in secretory granules was observed. Decrease in stainability was also seen in Periodic Acid-Schiff (PAS) stain and Azan stain. B. In the striated ducts, regressive changes such as atrophy, pyknosis, and deformation of epithelial cells and granular duct cells of striated ducts were seen. Especially in the granular duct cells, azocarmin chromatophilic secretory granules decreased or disappeared and stainability also decreased. C. These findings were markedly observed first in the salivary gland tissue adjacent to the extirpation wound and gradually in the surrounding salivary gland tissue also. Although most marked regressive changes were seen in the cases from 4 to 6 days after operation, changes were hardly seen in the whole submandibular gland. At the same time, many findings of disappearance of acinous cells due to atrophy, deformation, and destruction were seen, but the regressive changes such as atrophy and deformation in the epitherial cells and glanular duct cells of striated ducts were relatively slight and disappearance was hardly seen. D. In the cases from 7 days after operation, undifferentiated cells appeared in the epitherial cells of striated ducts and granular duct cells in which regressive changes had occurred. The cells showed with time cell nidi like acinous cells of the terminal portion and duct-like structures, gradually increased in regular arrangement, and formed relatively clear submandibular gland lobulus. In the cells like acinous cells of the terminal portion, secretory granules showing positive reaction in PAS stain gradually increased. III. On the basis of the foregoing experimental results, the wound by partial extirpation of rat submandibular gland underwent fibrous scar healing and the remaining salivary gland tissue was subjected to considerably severe regressive changes. However, it was suggested that repair and regeneration start from the remaining striated ducts and the injured salivary gland tissue recover morphologically and functionally. Repair or regeneration of salivary gland however was not observed in the defect by extirpation during this experiment.
- 九州歯科学会の論文
- 1992-12-25