破骨細胞の活性化に関する研究 : 破骨細胞の付着性の促進について
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概要
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This paper deals with the adhesiveness of the osteoclasts disaggregated from femora of 5-day-rat and the condition and factors affecting the adhesiveness during the incubation. The purpose is to show that the increase in osteoclastic adhesiveness is the first step of bone resorption. Results are as follows : 1. The cells disaggregated from femora of 5-day-old rats were sedimented for 15 min and incubated for 1 day on the slices of bovine cortical bone. Osteoclasts were identified as tartrate resistant acid phosphatase (TRAP)-positive cells. Many osteoclast adhered on the slices and many resorption pits occurred after incubation for 1 day. In the case of the bone slices washed after 15 min-sedimentation and incubated for 1 day, few osteoclasts adhered on the slices and no resorption pits occurred on the surface. These results show that the femora from 5-day-old rats contained many low adhesive osteoclasts which were removed by washing, and that the cells from 5-day-old rats increased the adhesiveness during the incubation. 2. Incubation media affected the adhesiveness of osteoclasts. After 1-day-incubation with medium 199 or with a-minimum essential medium, numbers of adhered osteoclast were higher than those with the other culture media. 3. Number of adhered osteoclast in the culture containing rat serum was very high in comparison with that in the culture without serum. Among sera from the other animals, only fetal bovine serum increased the adhered osteoclast number which was similar to that in the case of rat serum. 4. Parathyroid hormone (PTH) increased the number of adhered osteoclast, and the effect was a maximum at 10 ng per ml. Interleukin 1 (IL-1) also increased the number of adhered osteoclast at 10 U. The result that the increase in the adhesiveness by bone resorption activators PTH and IL-1 suggests that the increase in the adhesiveness is one of the activation process of the osteoclastic bone resorption. Thus it was shown that the many osteoclasts from 5-day-old rats were low adhesiveness and the adhesiveness was increased by some factor (s) in serum such as PTH and IL-1.
- 九州歯科学会の論文
- 1992-10-25