口腔領域悪性腫瘍患者血清が培養 KB 細胞におよぼす影響

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Early clinical diagnosis of oral cancer and determination of effect of treatment are possible under direct vision. For determination of insufficient control in deep tissue and metastasis, however, clinical examination routinly performed and tumor markers attracting attention recently are of reference. Organism with cancer, on the other hand, has tumor and host relationship. For the purpose of demonstrating correlation between examination findings and progress, therefore, serum of patients with oral cancer was added to KB cells derived from oral cancer in logarithmic phase in vitro and correlation between growth of KB cells and progress was examined. Whether this correlation can be used as a screening test was also examined. The results were as follows : 1. Patients with squamous cell carcinoma in oral cavity as compared with patients with benign oral disease showed significant decrease in erythrocytes, serum total protein, albumin, A : G ratio, and significant increase in alpha-1, 2-globulin, LDH, ALP, IgA and serum IAP level. In progress of clinical stage, statistically significant difference was found in each of erythrocytes, albumin, A : G ratio and alpha-1-globulin of Stage II cases ; erythrocytes, albumin, A : G ratio and alpha-1-globulin of Stage III cases ; erythrocytes, leukocytes, albumin, A : G ratio, alpha-1, 2, ganma-globulin, IgG and serum IAP level of Stage IV cases, as compared with patients with benign oral disease. 2. In change in measurements before and after treatment, with progress of clinical stage, no statistically significant difference was found in any measurement before and after treatment in Stage I and II cases. In Stage III cases, however, statistically significant difference was found in alpha-1, 2-globulin before and after treatment. Stage IV cases showed statistically significant difference in alpha-2-globulin before and after treatment. Positive correlation was found between growth of KB cells and alpha-1, 2-globulin. In contrast, negative correlation was found between growth of KB cells and the number of lymphocytes. 3. In cases with good prognosis, growth of KB cells was within or less than control level. In cases with poor prognosis, most cases were more than control level. Statistically significant difference was found between the cases with good prognosis and those with poor prognosis. In the measurements before and after treatment between the cases with good prognosis, statistically significant difference was found in albumin, A : G ratio and alpha-2-globulin. The cases with poor prognosis before and after treatment showed statistically significant difference in the number of lymphocytes, and alpha-1, 2-globulin. Of those, alpha-1, 2-globulin showed positive correlation with growth of KB cells. In contrast, the number of lymphocytes and A : G ratio showed negative correlation. 4. Serum IAP level before treatment in Stage III and IV cases increased significantly as compared with benign oral disease and Stage I cases. In cases with good prognosis, the level decreased significantly and tended to become negative, whereas in the cases with poor prognosis, the level increased significantly after treatment. From these, marked positive correlation was found between serum IAP level and growth of KB cells. 5. On the basis of the foregoing findings, reference to growth of KB cells, after adding serum of patients with oral cancer to KB cells and cultivating it, appears to reflect severity of condition in squamous cell carcinoma in oral cavity, and may be a useful indicator for estimating clinical course, effect of treatment, and prognosis, and may be used as a screening test.
九州歯科学会の論文
1992-02-25

口腔領域悪性腫瘍患者血清が培養 KB 細胞におよぼす影響

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