ジアゼパムとベンゾジアゼピン拮抗薬フルマゼニルの実験的投与による線条体アセチルコリン神経活動の変動 : マイクロダイアリシスによるラット線条体アセチルコリン神経での検討
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概要
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In vivo microdialysis was used to study the effcts of diazepam and flumazenil on extracellular concentration of acetylcholine in the striatum. Male Sprague-Dawley rats (n=23) were used as the experimental subjects, and were divided into three groups : the diazepam group (n=8), the diazepam + flumazenil group (n=7), and the saline group (n=8). In the diazepam group, rats were injected diazepam 5mg/kg intraperitoneal. In the diazepam + flumazenil group, rats were injected diazepam 5mg/kg and flumazenil 5mg/kg intraperitoneal. In the saline group, rats were injected saline 1ml/kg intraperitoneal. Extracellular concentration of acetylcholine was measured at fifteen minutes intervals. Before drug administration to the rat, microdialysis was carried out under a freely moving condition. Acetylcholine was quantified by a high performance liquid chromatography coupled with an electrochemical detector. The extracellular concentration of acetylcholine significantly decreased in the diazepam group. Basal extracellular concentration of acetylcholine was 31-32 pmol, and a minimum concentration was 24.0±12.9 pmol (mean±SD, p<0.001 versus basal concentration). In contrast, it didn't significantly affect the diazepam + flumazenil group. And there was no significant change in the saline group. In conclusion, diazepam reduces the release of acetylcholine and this effect was antagonized by flumazenil. These results suggest that diazepam reduces the acetylcholine neural activity in striatum, and this effect appears to be dependent of benzodiazepine/γ-aminobutylic acid receptor complex.
- 九州歯科学会の論文
- 1991-12-25