ラット顎下リンパ節マクロファージの分布と機能に関する研究

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In the mechanism of oral biophylaxis, macrophages have various functions. They perform a scavenging function at sites of tissue injury, play an essential role in the destruction of certain pathogenic organisms, and present antigens to lymphocytes. They are observed not only in periodontal tissue but also in drain lymph nodes. In the lymph nodes, macrophages exist in several compartments and they are morphologically different in each compartment. Macrophage populations exhibit heterogeneity with respect to morphology, biochemistry, secretory products and surface phenotype, but it is not known how much this heterogeneity reflects the existence of different stable lineages. So it has been hoped that monoclonal antibodies specific for macrophages or some macrophage subsets will provide a useful way to answer the difficult questions as to which cells belong to this lineage. In this study, three monoclonal antibodies recognizing the antigens expressed on macrophages were selected. After gingival injection of LPS, submandibular lymph nodes were taken out and their cryostat sections were made. The sections were observed after staining by the immunoperoxidase technique. The results were as follows : 1. Monoclonal antibody (mAb) ED 1 was useful in observing the distribution of general macrophages and dendritic cells in the cryostat sections of the rat submandibular lymph nodes. 2. It was suggested that mAb ED 3-positive cells in subsinus layer were the specialized macrophages, because these cells did not react with mAb ED 1 which recognized general macrophages. 3. The distribution of mAb OX-43-positive cells were far different from ED 1 and ED 3-positive cells. It was suggested that mAb OX-43 recognized a macrophage subpopulation that was quite particular. 4. Concerning the adherent cells isolated from submandibular lymph nodes, the largest number of the cells which had the phagocytic activity and possessed Fc and C 3 receptors on their surfaces appeared on the 1st day after gingival injection of LPS. The result probably reflected the property of the free macrophages in the lymph nodes. 5. Concerning the cells in the cryostat sections, on the 3rd day after gingival injection of LPS the largest number of the cells labelled with ED 1, ED 3 and OX-43 were observed and the degree of staining was the heaviest. It was suggested that on the 3rd day after gingival injection of LPS the macrophages in the submandibular lymph nodes were most activated and were actively phagocytizing the antigens and presenting them to lymphocytes.
九州歯科学会の論文
1990-10-25

著者

山本博久
九州歯科大学口腔細菌学講座

ラット顎下リンパ節マクロファージの分布と機能に関する研究

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