歯周炎の進行と補体活性化に関する研究 : 実験的歯周炎における補体活性化について : 実験的歯周炎における補体活性化について

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The endotoxin of Gram-negative bacteria has various biological activities and many studies show evidence to support this. Especially in the initial lesion in periodontitis, endotoxin plays an important part in complement cleavage. The aim of this study was to investigate changes with time in complement and immunoglobulins in the gingiva and serum by inoculating the gingiva of rats with endotoxin from E. coli. The results were as follows : 1. The immunofluorescent technique for locating C3 deposits in the periodontal tissue after LPS inoculation showed strong immunofluorescent stain in the blood vessel walls and the surrounding areas adjacent to the site of inoculation three hours after inoculation. The immunofluorescent stain decreased greately six hours after inoculation. 2. The values of C3 in the gingival extracts were about 3.8 times higher at three hours, about 1.2 times higher at six hours, and about 1.8 times higher at 24 hours than the normal value, and were about normal at 168 hours. 3. No marked changes in C3 were observed in the serum. 4. The values of C3_c in the gingival extracts were about 3.7 times higher at one hour, about 1.8 times higher at six hours, and about 3.4 times higher at 24 hours than the normal value, and were about normal at 72 hours. A diphasic change was seen in increase and decrease. 5. No marked changes were seen in C3_c in the serum. 6. No marked changes were seen in complement titer of serum (CH50). 7. As to immunoglobulins in the gingival extracts at one hour, IgG was 1.4 times higher, IgM 1.2 times higher, and IgA 1.1 times higher than the normal values, showing a slight increase. The values however were about normal in three to six hours. 8. No marked changes were found in whole IgG, IgM, and IgA in the serum. 9. With regard to anti-LPS immunoglobulins in the serum, no marked changes were seen for anti-LPS IgM and a definite increase was observed at 168 hours. A slight increase was seen for anti-LPS IgM at 72 hours and a definite increase was seen at 168 hours. On the basis of the foregoing, the infiltration of LPS into the tissue led the complement at site to an abrupt cleavage through the alternative pathway. The direct effect of LPS and indirect effect through cells and others resulted in a biphasic change in increase and decrease. However, the complement in the serum circulated through the circulatory system did not show any changes. Only anti-LPS immunoglobulins showed changes in 72∿168 hours.
九州歯科学会の論文
1987-12-25

歯周炎の進行と補体活性化に関する研究 : 実験的歯周炎における補体活性化について : 実験的歯周炎における補体活性化について

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