Differential Expressions of Laminin and Matrix Metalloproteinases in the Metastatic and Non-metastatic Cells Isolated from Squamous Cell Carcinoma (O-1N)

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Expressions of laminin and matrix metalloproteinases (MMPs) were investigated in metastatic and non-metastatic cells isolated from an experimental model of lymph node metastasis (O-1N). By Northern blot analysis, the metastatic cells expressed mRNA for laminin 6 days after plating, whereas the expression was very weak in the non-metastatic cells at the same stage. Immunohistochemical signals for laminin observed initially in the cytoplasm gradually increased in intensity and number with time and showed a honeycomb-like meshwork pattern at 7 days in both cells. The 92kD gelatinolytic activity (MMP-9) on zymography was stronger in the metastatic cells than in the non-metastatic cells. The 72kD band (MMP-2) was detected equally in both cells. The 59kD and 56kD caseinolytic activities (MMP-3) were observed only in the metastatic cells. The results indicate that the enhanced gene transcription and biosynthesis of laminin and concordant active destruction of basement membrane by MMPs in the metastatic cells may be involved in the process of lymph node metastasis in O-1N.
特定非営利活動法人日本臨床口腔病理学会の論文