流路反応を利用した第1, 2及び3アミンの同時検出法の開発

概要

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Simultaneous detection methods have not been developed with the exception of conductivity method connected ion exchange chromatography, although various detection methods corresponded to each amines have been needed for obtaining the pri-, sec-, and tert-amine moiety in structures. The author has developed three HPLC systems for the simultaneous detection amine compounds, based on online reaction. 1) Online derivatization and chemiluminescence reaction system; pri- and sec-amine compounds are derivatized to tert-amine compound(s) by epichlorohydrin, the following the tert-amine compound(s) is detected by chemilumenescence reaction of [Ru^<III>(bpy)_3]^<3+> and a CL detector. 2) An online oxidation and Griess reaction system; amine compounds are oxidized to nitrogen oxides by oxidation reagent and UV irradiation to promote of the reaction. And nitrite ions in the nitrogen oxides are used as a detection probe, and are detected by the Griess reaction. 3) Online oxidation reaction and OPA fluorescence reaction system; the nitrogen oxides generated from amine compounds by online oxidation are reduced to NH_3 on the reduction column packed with zinc powder. And the NH_3 as a detection probe is detected by OPA fluorescence reaction and a fluorescence detector. The simultaneous detection was proposed by these detection systems proposed in this study.
社団法人日本分析化学会の論文
2006-01-05