ニゲラン分解菌Arthrobacter sp. NHB-10株の分離と分離菌の生産するマイコデキストラナーゼの性質

概要

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A nigeran-degrading bacterium was isolated from soil and identified as Arthrobacter sp. (strain NHB-10). An enzyme hydrolyzing nigeran (alternating α-1,3-and α-1,4-linked glucan) was partially purified from the culture filtrate of strain NHB-10 by chromatographies on DEAE-Sepharose, Sephadex G-100 and DEAE-Sephadex A-50. THe enzyme preparation showed a single band with a molecular weight of about 70,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, but two bands with different isoelectric points of 7.80 and 7.40 on a thin-layer agarose gel for electrofocusing. The enzyme specifically hydrolyzed the nigeran into nigerose and nigeran tetrasacccharide by an endo-type action, indicating that it is a mycodextranase (EC 3.2.1.61) cleaving only the α1,4-glucosidic bonds in nigeran. The optimum pH and temperature for the enzyme activity were 6.0 and 50℃, respectively. The enzyme was stable in the pH range from 6.0 to 9.0 and up to 50℃.
社団法人日本生物工学会の論文
1996-01-25