842 In-vitro and in-vivo coupling of Thiocapsa hydrogenase to cyanobacterial and algal electron mediators

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概要

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• H_2-activation by the oxygen-resistent hydrogenase from the purple nonsulfur bacterium Thiocapsa roseopersicina, using electron mediators of cyanobacterial and algal origin, has been demonstrated. Ferredoxins, either from the cyanobacterium Synechococcus elongatus or the green alga Scenedesmus obliquus are capable of providing electrons for hydrogenase-mediated H_2-evolution, whereas ferredoxin is not reduced by Thiocapsa hydrogenase. Surprisingly, the high-potential cytochrome c_6 from Synechococcus elongatus proved to be capable of accepting electrons derived from hydrogenase-mediated H_2-oxidation. In subsequent experiments Thiocapsa hydrogenase was introduced into cells of Synechococcus elongatus by electroporation, leading to enhanced hydrogen production. In these experiments it has been demonstrated that hydrogenase from Thiocapsa roseopersicina is able to couple to the electron transport chain of Synechococcuss elongatus, justifying future efforts towards heterologous expression of this hydrogenase in Synechococcus elongatus.

• 1998-08-31

著者

• Miyake J.

  Nair/aist

• Miyake M.

  Nibh/aist

• Schnackenberg J.

  NIBH/AIST

• Asada Y.

  NIBH/AIST

関連論文

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