Electrophysiological Properties of Inhibitory Junction Potential in Murine Lower Oesophageal Sphincter

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概要

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• The electrophysiological properties of smooth muscle in the murine lower oesophageal sphincter (LOS) were investigated by intracellular microelectrode recording. Inhibitory junction potentials (IJPs) evoked by trains of field stimulation (30 V, 0.2-0.3 ms, 10 stimuli at 1-50 Hz) were observed in the murine LOS in the presence of atropine (1μuM) and nifedipine (1 μM). The IJP consists of two components, which we termed fast IJP and slow IJP. The fast IJP was partly sensitive to guanethidine (5 μM), pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid (PPADS, 30 μM) and apamin (0.1 μM), suggesting that the fast IJP was produced partly through the activation of apamin-sensitive Ca^<2+>-activated K^+ channels and of P_2-purinoceptors. The other part of the fast IJP was sensitive to N^ω-nitro-L-arginine (L-NNA, 100 μM) and 1H-[1,2,4] oxadiazolo [4,3-a]quinoxaline-1-one (ODQ, 1 μM), but insensitive to apamin (0.1 μM), iberiotoxin (50 nM) and charybdotoxin (30 nM). Slow IJP was sensitive to L-NNA (100 μM), ODQ (10 μM) and glibenclamide (10 μM), but insensitive to apamin (0.1 μM), iberiotoxin (50 nM) and charybdotoxin (30 nM). KT5823, a protein kinase G (PKG) inhibitor, had no effect on the fast and slow IJP in this tissue. It was suggested that, in the mouse LOS, adenosine trisphosphate (ATP) partly mediated the fast IJP through apamin-sensitive Ca^<2+>-activated K^+ channels, and nitric oxide mediated the remained part of the fast IJP and the slow IJP through cGMP, but not PKG. ATP-sensitive K^+ channels were suggested to be partly involved in the production of slow IJP, but the responsible channel (s) for the nitrergic fast IJP remained unclarified.

• 日本平滑筋学会の論文

著者

• Imaeda Kenro

  Department Of Gastroenterology And Metabolism Nagoya City University Graduate School Of Medical Scie

• Imaeda Kenro

  Department Of Internal Medicine And Bioregulation Nagoya City University Graduate School Of Medical

• CUNNANE Thomas

  Department of Pharmacology, University of Oxford

• Cunnane Thomas

  Department Of Pharmacology University Of Oxford

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