液体クロマトグラフィー/質量分析法の高感度化及び高分離能化に関する研究(博士論文要録)

概要

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The presented thesis has describes studies on the development of highly sensitive and selective LC/MS methods for the analysis of endocrine disruptors and mycotoxins. A highly selective LC/MS method for the analysis of OH-BaPs was developed. This method was successfully used for the analysis of metabolized samples by CYP1A1/epoxidehydrorase. A highly sensitive LC/MS and FL simultaneous detection method was developed. This method was successfully used to determine aflatoxins added to red pepper at a concentration of 10μg/kg. The systematic optimization of on-column focusing was established. With this work, improvements in the sensitivity at 30∿50 fold by on-column focusing will be routinely achieved. The mechanisms of a shoulder and split peaks appearing when large-volume samples were injected to a LC column were elucidated. A highly sensitive method for detecting trichothecens using LC/MS was developed. This technique, which monitors [M+Na]^+ and in which the mobile phase contains NaCl, has a great advantage of being insensitive to variations of the NaCl content in the sample solvent, and is more sensitive than other [M+H]^+ monitoring LC/MS methodologies. In conclusion, those developed methods are very useful for the analysis of endocrine disruptors and mycotoxins. In addition, those techniques will be applicable for other LC/MS methods.
社団法人日本分析化学会の論文
2005-04-05