ガスクロマトグラフィーによるマンノサミンの同定法

概要

論文の詳細を見る
A simple and accurate method for the identification of D-mannosamine in complex polysaccharides is described. The method is based on acid hydrolysis of complex polysaccharides, methyl glycosidation of the products with a cation exchange resin as catalyst, followed by acetylation, and isothermal gas-liquid chromatographic analysis of the derivatives. As the gas-liquid chromatographic column packing material, the low polar SE-30 was adequate for fractional detection of hexosamines from other contaminated components but was not so good for separation of each glycoside. On the other hand, the high polar Tabsorb^[○!R] and Silar-10C were rather good for separation of each glycoside one another. The method could be powerful means for the identification of mannosamine in complex polysaccharides, especially in trace amount of polysaccharide from microorganisms.
社団法人日本分析化学会の論文
1983-11-05