アルカリ性ペルオキシ二硫酸カリウム分解を用いる環境水中の全窒素測定法における硝酸イオンの紫外吸光光度定量法の応用

概要

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A simple and rapid procedure is presented for routine analysis of total nitrogen in environmental water samples. IC involves alkaline persulfate digestion and ultraviolet spectrophotometric determination of nitrate produced. Twenty ml samples are taken into 30ml Teflon bottles, and the bottles are closed with caps after addition of 3ml of 6%(w/v) potassium persulfate in 1.5M sodium hydroxide solution. After autoclaving at 121℃ for 30min, samples are filtered if necessary and acidified by adding 1ml 3.5M hydrochloric acid. The absorbance at 220nm or 230nm, depending upon the concentration of total nitrogen and the presence of interfering substances, is measured against the reagent blank using 1cm quartz cuvettes. The calibration curves of nitrate follow Beer's law up to 2mgN/l at 220nm and 10mgN/l at 230nm. Minimum detectable concentration is 0.05mgN/l. When ultraviolet spectrophotometric method was applied to the determination of total nitrate after digestion, there would be no interference by organic compounds.
社団法人日本分析化学会の論文
1981-10-05