p-ジエチルアミノシンナムアルデヒドを用いるピルビン酸の吸光光度定量法

概要

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The spectrophotometric determination of pyruvic acid with cinnamaldehyde derivatives, such as ρ-diethylaminocinnamaldehyde (DEAC), ρ-morpholhocinnamaldehyde (MC), ρ-nitrocinnamaldehyde, 3,4-dimethoxycinnamaldehyde, and 2-franacrolein, was studied. Pyruvic acid was found to react with DEAC and MC to give a red color in aqueoous ethanol solution in the presence of strong alkali. It was noticed that DEAC gave higher sensitivity and deeper coloration. The colored product with pyruvic acid showed a bathochromic shift with increasing sodium hydroxide concentration and reaction time, and a hypsochromic shift with increasing reaction temperaturre. The calibration curve followed Beer's law in the range of (0〜0.4)mM pyruvic acid. The coefficient of variation is about 2.2% at 0.2 mM pyruvic acid. To 1 ml of sample, 1 ml of 10 N sodium hydroxide solution and 3 ml of 0.4w/v% DEAC-ethanol solution were added. The mixture was kept for 25minutes in a water bath at 40℃. After cooling in water, the absorbance was measured at 495nm agaist a reagent blank. The effects of α-ketoglutaric acid and dl-alanine on the absorbance were examined. The procedure is useful for the assay of enzymes which are concerned with pyruvic acid.
社団法人日本分析化学会の論文
1975-02-10