ヘキサシアノ鉄(III)酸イオンをメディエーターとする定電位クーロメトリーによる還元型β-ニコチンアミドアデニンジヌクレオチドの絶対定量

概要

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Controlled potential coulometry with carbon felt electrodes has bee absolute determination of reduced nicotinamide-adenine dinucleotide (NADH) using hexacyanoferrate(III) ion and vitamin K_3 mediators. The reduced forms of mediators produced by the NADH addition are completely oxidized to original oxidized forms at the working carbon felt electrode impregnated with electrolyte containing mediator. The oxidation of vitamin K_3 by NADH takes place rapidly when diaphorase is contained in an electrolyte. But, the reduced form of vitamin K_3 undergoes air oxidation during electrolysis and the current efficiency of NADH obtained by using vitamin K_3 mediator was then below 90%, consequently, the absolute determination of NADH could not be carried out by using vitamin K_3 mediator. On the other hand, an absolute coulometric determination of NADH were possible when hexacyanoferrate(III) ion mediator is used, because the oxidation rate of NADH by high concentration hexacyanoferrate (III ) ion (>0.1 M) is sufficiently high, even when diaphorase is not used. Moreover, the produced hexacyanoferrate(II) ion by the reduction of hexacyanoferrate(III) ion by NADH is stable to air oxidation and the current efficiency of NADH was found to be attained more than 99 %.
社団法人日本分析化学会の論文
1993-05-05