段階的流量比法/非相分離吸光光度検出による分配係数の測定

概要

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A new system of flow ratiometry having UV detection without a phase separator was developed and applied to the determination of the chloroform/water distribution coefficients (K_D) of phenol and its derivatives. An aqueous solution of analyte and chloroform was delivered independently and merged at various now ratios (R_f). The ratio was changed stepwise. Both phases, segmented each other, passed through an extraction coil and were directly led to a flow cell, where the absorbance of each phase was monitored. A wide-bore tubing (2 mm, i.d., 20 cm long) was inserted between the extraction coil and the flow cell in order to coalesce the chloroform segments and/or aqueous segments. Thus formed bigger segments were easily measured. The K_D was calculated from the slopes and the intersections of linear plots of A^<-1> vs. R_f, (AR_f)^<-1> vs. R_f^<-1>, and AR_f vs. A, where A is A_o (absorbance of chloroform phase), A_a(absorbance of aqueous phase), ΔA (= A_o - A_a) or ΣA (= A_o + A_a). Among them, the plots using ΣA as A gave the best results, probably due to a cancellation of the intrinsic errors in the A_o and A_a measurements. The method was simple and efficient (<5 min/determination) with satisfactory precision.
社団法人日本分析化学会の論文
2004-04-05