ポストカラム誘導体化に電極反応を利用した高速液体クロマトグラフィー

概要

論文の詳細を見る
A new technique of on-line electrochemical derivatization using glassy carbon column electrode as a reactor is established, Materials eluting from a separation column are converted in the reactor to form fluorescent or intensely UV-absorbing products, the products being monitored by a suitable detector. An example of the use of this technique is the detection of homovanillic acid(HVA) in urine. HVA is applied to the column (Hitachi #3011-N, 250×4mmi.d.) of HPLC and derivatized to the fluorescent dimer (2,2-dihydroxy-3,3-dimethoxybiphenyl-5,5-diacetic acid) by electrochemical oxidation in the reactor. The intensity of the fluorophore is measused with a spectro-fluorometer (λ_<ex>325nm, λ_<em>420nm) and reorded graphically. Linearity of the relationship between pcak hight and amount of HVA is obtained between 5 ng and 120 ng. The reproducibility, expressed in terms of coefficient of variation, is 1.6%(20ng, n=1O). As none of the derivatizing reagent need to be added to eluent stream, this technique has a little or no effect on the dilution of eluate, back ground noise based on the derivatizing reagent and peak broadening. This technique will extend to many substances.
1983-02-05