カルニチンに関する研究 : (VI)種々のビタミンおよびアミノ酸を含む製剤中のカルニチンの定量

概要

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Carnitine in preparations, not containing any other trimethyla mmonium derivatives can precisely, be determined within 2 to 3 hours as follows : Samples are heated in 75% KOH solution at 150℃ to decompose repidly carnitine quantitatively to trimethyla mine. The trimethyla mine thus formed is trapped and measured colorimetrically with the phenol reagent of Folin and Ciocalteu. Carnitine in a solution in which it is the only substance that precipitate with Reinecke salt may precisely be determined within 1 1/2 hours by adding an excess amount of Reinecke salt solution to the solution, allowing to stand to complete the precipitation, filtering the mixture, and determining the excess Reinecke salt colorimetrically. Such interferes as thiamine, choline, etc. are completely removed by passing through an Amberlite CG-120 column buffered at pH7.0 without the loss of carnitine.
日本ビタミン学会の論文