ビタミンB_<12>の腸管吸収に必要な受容体に関する研究 : 特に小腸粘膜上皮細胞における分布

概要

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One and half hours after feeding of ^<57>Co-vitamin B_<12> to rats, small intestines were extirpated. Using Weiser's method, gradient isolation of the intestinal epithelial cells from villus to crypt areas were carried out and five different levels of the villus and crypt areas were obtained. The uptakes of ^<57>Co-vitamin B_<12> and the formations of Receptor-Intrinsic factor-^<57>Co-vitamin B_<12> complex were measured, indicating that the upper villus cells have a higher uptake of ^<57>Co-vitamin B_<12> and more increased formation of it than crypt cells do showing a gradient from crypt to villus. Alkaline phosphatase activity of these levels of epithelial cells also showed the same pattern. These results suggest that the receptor activity for vitamin B_<12> absorption in the intestinal epithelial cells indicate a gradient increase from crypt to villus areas.
日本ビタミン学会の論文
1977-01-25