ストレプトマイシン生産とムコペプチド生合成の関係

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When washed mycelia of Streptomyces griseus HUT 6037 were suspneded and shaken in 0.5% NaCl solution, streptomycin appeared in the supernatant. Addition of glycine, L-serine, L-phenylalanine, L-threonine, or L-lysine in 2% concentration to the suspension culture increased streptomycin production. L-Phenylalanine had the highest stimulating effect among these five amino acids. These amino acids, however, depressed incorporatini of L(U-^<14>C)alanine into mucopeptide in the above suspension culture, suggesting that streptomycin production was closely correlated with mucopeptide synthesis in the cell wall.In short term experiments on mycelium-suspended culture, the effects of phenylalanine and antibotics which were effective inhibitors of cell wall synthesis were examined on the incorporation of D-glucosamine into the cell wall and streptomycin. When phenylalanine, bacitracin, or enduracidin was added together with D-(U^<14>C)glucosamine as a tracer, incorporation of D-glucosamine into mucopeptide decreased, but that into streptomycin increased, suggesting that there was an alternative route for D-glucosamine incorporation. This alternative used of D-glucosamine was also observed, though to a lesser extent, in a long term experiment with the mycelium-suspended culture without either amino acid or antibiotic.When both ^<14>C-glucosamine and bacitracin were added simultaneously to the suspension culture, about 60% of the labeled carbon incorporated into streptomycin was located in the N-methyl-L-glucosamine moiety.
公益社団法人日本生物工学会の論文
1976-08-25

ストレプトマイシン生産とムコペプチド生合成の関係

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