清酒酵母からのKiller耐性変異株の分離およびその諸性質

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In the previous paper, in was reported that all of sake yeasts were sensitive to killer strains. Isolation of killer-resistant mutants from sake yeast was then investigated and three strains were obtained. Killer-resistant mutant, strain No. 701X-2,did not form an inhibition zone by well test and the other two strains, petite-like colony mutants, formed a smaller zone (1/30-1/10 that of their parent strains). In te mixed culture of sake yeast and killer strain No. 78,the death of the former was delayed or prevented by changing the rato of inoculum size of the two strains, by the kind of nitrogen source in the medium and by the addition of protease or rice koji to the culture medium. When strain No. 701X-2 and strain No. 78 were utilized in the mixed culture with an inoculum size ratio of 15 to 1 respectively, normal growth of strain No. 701X-2 was observed. However, the parent strain No. 701 was killed, even when its inoculum size was 70 times than that of strain No. 78. When beef extract was used as nitrogen source in the modified Lodder's medium, strain No. 701 was killed but strain No. 701X-2 grew a little in mixed culture with strain No. 78. Conversely, strain No. 701 showed slight growht and strain No. 701X-2 grew more than strain No. 78 with (NH_4)_2SO_4 or casamino acid as nitrogen source.
社団法人日本生物工学会の論文
1974-05-25