菌体内核酸の自己分解による5′-ヌクレオチド生産に関する研究 : (第1報)Candida utilisによる5'-ヌクレオチド分泌に対するホヴ酸塩および界面活性剤の影響

概要

論文の詳細を見る
The conditions of the excretion of 5'-nucleotides due to the degradation of endogenous ribonucleic acid (RNA) of a strain of Candida utilis were studied. It was found 5'-nucleotides scarcely excreted by this organism when its intact cells were incubated in a buffer solution with acetate, phosphte or glycine as a buffer with various values of pH. It was also found that 5'-nucleotides were excreted by the organism when incubated in a glycine buffer containing a certain surfactant with an alkaline pH. Surfactants useful for this activity were common cationics and nonionics having ether bonds. But under these conditions, the yield of 5'-nucleotides was not enough. On the other hand, borate buffers were found to be very useful for the excretion of 5'-nucleotides without adding any surfactant Moreover, an addition of a surfactant under the presence of borate buffers worked out a complete degeneration of endogenous RNA into 5'-nucleotides and all the 5'-nucleotides formed were excreted outside the intact cells. The optimum conditions for the 5'-nucleotides excretion by the intact cells when incubated at 33℃ for 4 to 6 hrs., were as follows : 1) optimum pH 10.0〜10.3 2) boric acid or borate 0.1〜 0.25M 3) surfactant concentration 5〜10% to dry cells. Under these conditions, the autolysis occurred specifically in endogenous RNA and other cell components with a high molecular weight were remained substantially unchanged.
社団法人日本生物工学会の論文

菌体内核酸の自己分解による5′-ヌクレオチド生産に関する研究 : (第1報)Candida utilisによる5'-ヌクレオチド分泌に対するホヴ酸塩および界面活性剤の影響

スポンサーリンク

概要

著者

関連論文

スポンサーリンク