Clostridium saccharoperbutylacetonicumのバクテリオファージに関する研究 : (第9報)カナマイシンの抗HMファージ作用

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The action of antibiotic kanamycin on HM 2-phage of Clostridium saccharoperbutyl-acetonicum, a butanol-producing bacterium, was studied as a part of a basic study for preventing phage infection in industrial fermentation.Kanamycin showed selective inhibition of phage growth. Less than 200 μg/ml failed to inhibit the growth of uninfected host bacteria, whereas 200 μg/ml inhibited 99% of phage growth. It also inactivated the free phage particles (200 μg/ml inactivated 75% of phage in 4-hr incubation) and prevented the lysis of infected cells.Kanamycin (even 1000 μg/ml) did not inhibit the synthesis of DNA and protein in infected cells. Active phages were not detected when the lysis-inhibited cells were artificially lysed with chloroform. It appears to be likely that kanamycin inhibits the intracellular phage growth by preventing the maturation step.Kanamycin inhibited also the growth of HM 3-phage.Fradiomycin (neomycin), a similar group to kanamycin, also showed selective inhibition of the growth of HM-phages.