細菌のUricaseに関する研究 : (第2報)粗酵素の抽出および性質

スポンサーリンク

概要

• 論文の詳細を見る

• It has been reported in a previous paper that two bacteria, Micrococcus varians and Brevibacterium vitarumen var. uricum were isolated as uricase producers, and some conditions of the enzyme production were investigated. In this paper an uricase (an endoenzyme) extraction method and some general characteristics of crude bacterial uricase were investigated.As ultrasonic extraction is difficult to apply for industrial purpose, other extraction methods were tested. For strain Micrococcus varians a freezing and thawing method using dry-ice followed by extraction with phosphate buffer solution showed a 60% yield. The best yield was obtained with a phosphate buffer pf pH 8.0〜9.0 for 24 hours at 25℃.Strain Brevibacterium vitarumen var. uricum was found to have the ability to accumulate uricase in the broth when cells were incubated in the basal medium added with 0.1〜1.0% nonionic surfactant. Among 10 nonionic surfactants tested, Nikkol-R-2020 (a condensation product of polyoxyethylene octylphenol and formal-dehyde), polyoxyethylenelaurate and Nikkol HCO-60 (polyoxyethylene castor oil derivative) were found to be most effective. Induced external production of uricase by nonionic surfactant required a carbon source added to the medium, and only carbon sources capable to support the bacterial growth was found to be effective.Some properties of the crude uricase preparations were examined. The crude uricase was obtained either by using (NH_4)_2SO_4 between 0.2-0.6 saturation (Micrococcus varians) or acetone precipitation with acetone (Brevibacterium vitarumen var. uricum).The optimum pH for acitivity was found to be 8.5-8.8 with either enzyme and it had optimum stability at 9.0 (Microccus varians) and 8.0 (Brevibacterium vitarumen var. uricum) respectively. Both enzymes showed similar behavior to inhibitors. KCN, PCMB and hydroxylamine inhibited them seriously but EDTA and heavy metal ions had only minor effects. Reducing agents such as cysteine and ascorbate stimulate the enzyme reaction.

• 社団法人日本生物工学会の論文
• 1966-11-25

著者

• 国久 昌弘

  小野薬品工業株式会社中央研究所

• 白石 節子

  小野薬品工業株式会社

• 国久 昌弘

  小野薬品工業株式会社

• 木田 準之助

  小野薬品工業株式会社

• 木田 準之助

  小野薬品工業kk 中央研究所

関連論文

• カワラタケ由来ATSOの生体免疫機能に及ぼす影響
• Chemical Structure of Antitumor Polysaccharide, Coriolan, produced by Coriolus versicolor
• Coriolus Versicolor菌糸体産生抗腫瘍性多糖D-IIに関する研究
• 細菌のUricaseに関する研究 : (第2報)粗酵素の抽出および性質
• 細菌のUricaseに関する研究 : (第1報)Uricase生産菌株の分離と培養
• 細菌のuricaseに関する研究 : (第3報) Uricase生産菌Brevibacterium vitarumen var. uricumのadenineによる生育阻害と, そのuricase生成への影響
• 細菌のUricaseに関する研究-1・2-

スポンサーリンク