筍におけるリグニンの生成について(第III, IV報) : 筍組織片による前驅物質からのリグニンの形成

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1. By using 5 species of coniferous, 10 species of deciduous and 3 species of bamboo wood meals, the Maule's and Cl_2-Na_2So_3 reactions were examinde, and the absorption spectra of their colors were measured.In the case of coniferous wood, the absorption peak near 390 mμ was appeared in both reactions, whereas two absorption peaks near 390 mμ and 510 mμ were appeared with deciduous wood and the absorption spectrum of bamboo was similar to that of deciduous wood.2. From the above results, the authors assumed that the absorption peak near 390 mμ was responsible for the guaiacylpropane component, whereas the other absorption peak near 510 mμ was caused by the syringylpropane component of the lignin, and then both reactions were examined with some guaiacyl, syringyl and p-hydroxyphenxl compounds. Consequently, on the basis of absorption spectrum and stability of color, syringaldehyde, syringic acid and gallic acid gave the color characteristics of deciduous wood-their absorption peaks were near 510 mμ-whereas guaiacyl compounds such as vanillin, ferulicacid and coniferylalcohol gave only absorption peak near 39 mμ.3. About 1 kg of each wood meal was chlorinated, or treated with 1% KMnO_4 and then with 12% HCl respectively and thereafter each lignin preparation which gave positive color reaction was extracted by cold ethanol and was purified. Both lignin preparations were similar at following points : a) The contents of OCH_3,and Cl_2. b) Solubility and ultraviolet absorption spectrum. C) Both vanillin and syringaldehyde were found at the alkaline nitrobenzene oxidation.However, As a difference between both preparation was found on the stability of color which appeared for alkali, it was suggested that there were some different point of structure in detail.4. From the above results it was suggested that both reactions were due to the existence of syringyl nucleus contained in the lignin, and then the building unit of the lignin could be classified by these reactions.Accordingly, the lignin formation was examined by these reactions on the incubation experiment of embryonic root tip of kidney bean and bamboo-shoot tissue in some phenylpropane substances.5. As SIEGEL's experimental results showed, the formation of the substance which gave positive color reaction was accelerated by the addition of H_2O_2 and phenylpropane substance, and the formation of this substance was inhibited by some inhibitors of heavy metal containing enzyme, such as NaN_3,NH_2OH, CH_2ICOOH, H_2S and NO, as well as KCN and auxin. Then, it was sugested that the peroxidase was responsible to the lignin formation.6. No syringaldehyde was found in alkaline nitrobenzene oxidation of incubated sample with guaiacylpropane substances, whereas in the case of incubated sample with syringylpropane substances, both Maule's and Cl_2-Na_2SO_3 reactions became poitive and syringaldehyde was found in alkaline nitrobenzene oxidation. Accordingly, it was suggested that the formation of syringyl nucleus was occurred at the earlier stage than the formation of phenylpropane nucleus.7. Euenol and dihydroconiferylalcohol were not transformed to the lignin by the isolated peroxidase or phenoloxidase, whereas FREUDENBERG was found that the coniferylalcohol was transformed to the lignin by the isolated phenoloxidase or peroxidase. Then it was suggested that the eugenol and dihydroconiferolalcohol were oxidized to the coniferylalcohol type and then it was transformed to the lignin by the phenoloxidase and or peroxidase action.
日本森林学会の論文
1954-09-25

筍におけるリグニンの生成について(第III, IV報) : 筍組織片による前驅物質からのリグニンの形成

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