レツドキヤベツ抽出液による木材腐朽菌の診断法について

概要

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BAENDAMM's reaction has been used for diagnosis of wood-rotting fungi. According to the results of recent studies, it has been clear that there are two types of phenol oxidases (laccase- and tyrosinase-types) and that the substantial difference between white rot and brown rot fungi lies in that the former will secrete laccase.For laccase test, α-naphtol gives complete results, and p-cresol for tyrosinase test. But BAVENDAMM's reaction approximately coincides with the results of laccase test, based on the quantitative relation of both types of enzyme and the difference of their reaction velocity, etc.Recently, JφRGENSEN and VEJIBY have reported a new method for diagnosis of wood-rotting fungi in which red cabbage extract was used as chemical reagent. The writer rescrutinized this method and it was made clear that the reaction changing the colour of red cabbage extract yellow from purple with white rot fungi fairly coincided with the existence of laccase, and red from purple with brown rot fungi was based on strong acidity of media on which they developed vigorously.Since this colour reaction requires relatively short period and gives a result clearly, it is recognizable that this is an excellent method for diagnosis of wood-rotting fungi as belonging to the white or brown rot fungi.
1954-03-25