高度好熱性嫌気性細菌のセルロース分解特性

概要

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Cellulase production, insoluble cellulose digestion and growth of an extremely thermophilic cellulolytic anaerobe, strain NA10 (optimum temperature, 73℃), were studied in continuous cultures at different hydraulic retention times, keeping the influent cellulose concentration constant at 10 g/l. Most of cellulase produced by strain NA 10 attached to cell mass and cellulose. Cellulase activity in the culture broth was 0.07〜0.1 unit/ml, while cellulase productivity increased with an increase in retention time until nearly the washout point with a maximum productivity of 8.1 unit/l・h. The yield of acetic acid, the main product of cellulose digestion, was 32〜40% based on the carbon balance. Data of continuous culture in the steady state were analyzed and the following kinetic parameters for cellulose digestion by strain NA10 were obtained : Maximum specific growth rate=0.13 h^<-1>, maximum specific cellulose consumption rate=2.0 mg glucose equivalent/mg cell・h, saturation constant=200 mg cellulose/l, true growth yield=0.08 mg cell/mg glucose equivalent and maintenance coefficient=0.46 mg glucose equivalent/mg cell・h.
社団法人日本生物工学会の論文
1992-11-25