ビール粕を利用した微生物酵素の生産

概要

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Brewers' spent grain was used as the raw material for production of three extracellular enzymes, α-amylase, alkaline protease, and lichenase, by Bacillus licheniformis. The basal medium was composed of wet spent grain (about 80% moisture) and 40 mM phosphate buffer, pH 7.0 (1 : 5-10,w/v). For α-amylase production by strain no. 18,0.5% corn oil was added to the basal medium, and the maximum activity (66 units/ml) was found in the culture solution of the 4th day of incubation at 45℃. Alkaline protese from strain H-9 was producted in the basal medium supplemented with 2 mM CaCl_2 and 10 mM sodium citrate. The maximum activity during incubation at 35℃ was 7.2 units/ml on the 4th day. On the other hand, lichenase was produced sufficiently in the basal medium by strain Y-25,and the activity reached the level of 20 units/ml on the 3rd day of incubation at 30℃. This enzyme preparation could also hydrolyze β-glucans from malt and beer.
社団法人日本生物工学会の論文
1985-01-25